Relative independence of luminol-enhanced intensity of photon emission during oxidative burst from nondiluted human blood on the volume and surface area of the sample

摘要

Parameters of chemiluminescence (CL) from nondiluted human blood were studied. Kinetics and intensity of CL depended upon donor's state of health, time after blood extravasion, conditions of its storage, conditions of its counting. Peculiar dependence of Luminol-enhanced CL on sample volume changes during respiratory burst (RB) was revealed. When 0.5 ml aliquots were consecutively taken from blood and transferred into another vial of the same configuration, each subtraction of blood was followed by an acceleration of CL intensity growth. Summation of portions of blood in the second vial resulted in deceleration of CL intensity increase from it. At equal volumes of blood CL intensity from the first sample was manifold higher than from the second one and this difference was increasing on with further transfers. When blood was transferred back to the first sample, CL intensity from the 'donor' sample began to increase at a faster rate, while CL intensity from the 'recipient' sample stabilized. Such behavior was characteristic of nondiluted healthy donors' blood. Diluted blood or blood of sick people demonstrated different behavior. It is suggested that CL parameters of nondiluted blood may be informative of integrative properties of blood tissue.