Onsite VFA Production to Support Simultaneous EBPR and Denitrification- Role of Novel PAOs

摘要

Nutrient contamination has led to the impairment of various surface water bodies. Therefore, the overall efficient treatment of wastewater is crucial in order to maintain the quality of surface water. The current research was conducted to test a relatively novel wastewater treatment configuration. A pilot plant was constructed and operated in three different operational phases: a) Conventional A20 (Anoxic/Anaerobic/Oxic), b) extended HRT Westside RAS fermentation, and c) Westside RAS fermentation mode. External additional carbon was supplied through external RAS fermentation. The final effluent lowest concentration of <1 mg L~(-1) PO_4~(3-)-P was achieved, indicating the successful removal of biological P. Ammonia and total nitrogen removal were mostly consistent in all 3 phases, ppk gene copy number varied with the VFA feeding regime, and a change in copy number was observed when the fermentate feeding was intermittent, ppk and GAO copy numbers were inversely proportional to each other, indicating a positive competition for resources in the bioreactor. High throughput sequencing targeting the V4 hypervariable region of the 16S rRNA gene revealed the presence of Intrasporangiaceae under Actinobacteria phylum in high abundance.