Improved nucleus segmentation process based on knowledge based parameter optimization in two levels of voting structures

摘要

Digital analysis and biomedical image processing has become important part within modern medicine and biology. Digital pathology is just one of many medicine areas that is being upgraded by constant biomedical engineering research and development. It is very important that some of disciplines as nucleus detection, image segmentation or classification become more and more effective, with minimum human intervention on these processes, and maximum accuracy and precision. Improved optimization of nucleus segmentation methods parameters based on two levels of voting processes is presented in this paper. First level includes hybrid nucleus segmentation based on 7 segmentation algorithms: OTSU, Adaptive Fuzzy-c means, Adaptive K-means, KGB (Kernel Graph Cut), APC (Affinity Propagation Clustering), Multi Modal and SRM (Statistical region merging) based on optimization of algorithms parameters along with implemented first level voting structure. Second level voting structure includes segmentation results obtained in the first level of voting structure in combination with 3rd party segmentation tools: ImageJ/Fiji and MIB (Microscopy Image Browser). A definite segmented image of a nucleus could serve as a generic ground truth image because it is formed as a result of a consensus based on several different methods of segmentation and different parameter settings, which guarantees better objectivity of the results. In addition, this approach can be used with great scalability on 3D-stack image datasets.