DESIGN, CONSTRUCTION AND APPLICATION OF E. COLI/C. GLUTAMICUM SYNTHETIC CONSORTIA

摘要

In the biorefinery concept renewable feedstocks are converted to a multitude of value-added compounds irrespective of seasonal or other variations of the complex biomass substrates. On the one hand, this can be realized by specialized single microbial strains. Alternatively, consortia of several microorganisms or strains can be used. The latter approach allows for modularity, e.g. as various strains for substrate conversion can be combined with various strains for product formation. We have used E. coli for access to carbon sources (Wendisch et al.) and C. glutamicum for production of value-added compounds () in order to address the construction of binary microbial consortia based on starch- and sucrose-based production of L-lysine and derived value-added compounds (Sgobba et al. 2018). A commensalism-based synthetic consortium for L-lysine production from sucrose was developed combining an l-lysine auxotrophic, naturally sucrose-negative E. coli strain with a C. glutamicum strain able to produce L-lysine that secretes fructose when grown with sucrose due to deletion of the fructose importer gene ptsF. Mutualistic synthetic consortia with an L-lysine auxotrophic, a-amylase secreting E. coli strain and naturally amylase-negative C. glutamicum strains was implemented for production of valuable fine chemicals from starch.