Detecting De Novo Plasmodesmata Targeting Signals and Identifying PD Targeting Proteins

摘要

Subcellular localization plays important roles in protein's functioning. In this paper, we developed a hidden Markov model to detect de novo signals in protein sequences that target at a particular cellular location: plasmodesmata. We also developed a support vector machine to classify plasmodesmata located proteins (PDLPs) in Arabidopsis, and devised a decision-tree approach to combine the SVM and HMM for better classification performance. The methods achieved high performance with ROC score 0.99 in cross-validation test on a set of 360 type I transmembrane proteins in Arabidopsis. The predicted PD targeting signals in one PDLP have been experimentally verified.