COMBINING SINGLE-USE BIOREACTOR TECHNOLOGY AND TIPS METHOD TO MAKE IC/BEVS-BASED PRODUCTIONS MORE EFFICIENT

摘要

Insect cells (ICs) in conjunction with the baculovirus-expression vector system (BEVS) are a suitable production system for the development and manufacturing of recombinant protein products such as virus-like particle vaccines. In order to speed up vaccine development and production, and to react more rapidly to pandemic scenarios, new strategies for IC/BEVS-based production technologies need to be developed and transferred. Among these is the implementation of single-use (SU) bioreactors, which have been proven as cost-effective (48 % less capital costs), ready-to-use systems without time-intensive cleaning and sterilization processes when compared with reusable bioreactors [1]. A further increase in process efficiency can be achieved by using the titerless infected-cell preservation and scale-up (TIPS) method. Here, the infection is directly performed with baculovirus-infected insect cells (BIICs), which makes laborious, time-consuming virus determinations and virus amplifications obsolete, while providing product titers and product activities comparable to the standard method, the classical infection of non-infected ICs [2, 3].