Fabrication of a Free Radical Scavenging Nanocomposite Scaffold for Bone Tissue Regeneration

摘要

Porous nanocomposite scaffolds with active free radical scavenging agents can be successfully fabricated. Scaffold mechanical properties were anticipated due to the elastomeric matrix. EDS and Raman spectroscopy confirmed CNPs at the scaffold surface. H_2O_2 decomposition into water was accelerated in PTC20 scaffolds in comparison to PT scaffolds. MC3T3s exposed to H_2O_2 were better protected on PTC20 scaffolds, resulting in a lower relative cytotoxicity. Scaffolds were determined to be biocompatible, due to the ability to host cell attachment and high cell viability. CNPs can effectively provide protection from oxidative damage through free radical scavenging. Future steps include investigating changes in mineralization behavior and gene expression in osteo-type cells when exposed to ROS and FRS agents.