Electrophysiological Detection of Cortical Neurons under Gamma-Aminobutyric Acid and Glutamate Modulation Based on Implantable Microelectrode Array Combined with Microinjection*

摘要

Understanding the relationships between different cortical neurons under excitatory or inhibitory modulation is important for researches of many neurological disorders. In order to monitor the neural activities in cortex under gamma-aminobutyric acid (GABA) and glutamate (Glu) modulation, an implantable microelectrode array (MEA) was combined with microinjection capillary. The neurons in motor cortex of rat were modulated by GABA and Glu injection, and multichannel neural electrophysiological signals were simultaneously recorded. Spike analysis showed that the interneurons recorded by the MEA were inhibited after GABA injection and excited after Glu injection, but one pyramidal neuron was found to be not sensitive to the drug. The local field potentials (LFP) were most affected in the frequency band of 5~10 Hz after Glu injection, which greatly increased the amplitudes of the spindle-like waveforms. The MEA combined with microinjection provided a low-cost and effective tool for neurological drug modulation and evaluation in brain tissue.