In Situ Characterization of Superficial Organic Composition Changes of Thermoacidophiiic Archaea Acidianus manzaensis YN-25 in Response to Energy Substrate

摘要

The differences of superficial (about 10 nm) extracellular polymeric substances (EPS) composition of thermoacidophiiic archaea Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS_2, CuFeS_2, S°, FeSO_4) were analyzed in situ for the first time by synchrotron radiation (SR) based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy. The results showed that there are clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (-C=0, -C-0 and -C-N). The archaea acclimated with chalcopyrite and pyrite contain higher proportion of proteins but less polysaccharides compared to cells acclimated with S°. The archaea acclimated with FeS04 contains the highest proportion of protein which is positively associated with the iron oxidation process, while archaea acclimated with S° contains more lipids and polysaccharides, which may be favorable to the hydrophobic S° adsorption and utilization process.