Immobilization of gelatin onto acrylic acid grafted bacterial cellulose for regulating cell behaviors using gamma-irradiation

摘要

Bacterial cellulose(BC) scaffolds are generated from gluconacetobacter hansenii TL-2C. BC has been shown to have a high-burst pressure and ultraflne highly nanofibrous structure similar with that in a natural extracellular matrix (ECM) for tissue engineering. In this study, acrylic acid(AAc) was grafted onto BC surface using gamma-irradiation and then gelatin immobilized it using EDC/NHS reaction. The characterization of the scaffolds was performed by scanning electron microscopy, ATR-FTIR spectroscopy, a toluidine blue 0 assay, and 2,4,6,-trinitro-benzensulfonic acid assay. The surface morphology of scaffolds was confirmed BC membranes by SEM for gelatin and AAc grafted it, so that it did not change surface morphology. The adhesion and spreading of human mesenchymal stem cells was improved on the gelatin-AAc-BC nanofiber compared to unmodified BC and AAc-BC nanofiber. Therefore, gelatin and AAc grafted BC scaffolds have been possible to tissue scaffold that accelerate the cell behavior for biomedical applications.