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Opto-Plasmonic Biosensors for Monitoring Wheat End-Products Quality

机译:用于监测小麦最终产品质量的光电生物传感器

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The aspect of food quality is of increasing interest for both consumers and food industry. Detection of chemical contaminants and specific quality-related components are required. In this sense, biosensors can play a prime role, because these devices allow cheap and continuous monitoring of contaminants along the pasta production chain and are also useful to identify allelic variants of genes coding for protein associated to bad or good quality of wheat end-products. In this work we report the production of a whole-cell biosensor based on bacterial cells genetically modified to respond to the presence of metal ions by fluorescence or luminescence emission, and of a biosensor based on Localized Surface Plasmon Resonance (LSPR), that is able to detect nucleic acid sequences characterizing different allelic variants.
机译:食品质量方面越来越受到消费者和食品工业的关注。需要检测化学污染物和与质量相关的特定组件。从这个意义上讲,生物传感器可以发挥主要作用,因为这些设备可以廉价,连续地监控​​面食生产链中的污染物,并且还可以用于识别编码与小麦最终产品的品质不良或品质相关的蛋白质的基因等位变体。在这项工作中,我们报告了一种基于细菌细胞的全细胞生物传感器的生产,该细菌细胞经过基因修饰以通过荧光或发光发射对金属离子的存在做出响应,以及基于局部表面等离子体共振(LSPR)的生物传感器的生产,该传感器能够以检测表征不同等位基因变体的核酸序列。

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