COMPARISON OF SUBMERGED AND UNSUBMERGED PRINTING OF OVARIAN CANCER CELLS

摘要

A high-throughput cell based assay would greatly aid in the development and screening of ovarian cancer drug candidates.Previously, a three-dimensional microfluidic printer that is not only capable of controlling the location of cell deposition, butalso of maintaining a liquid, nutrient rich environment to preserve cellular phenotype has been developed (WasatchMicrofluidics). In this study, we investigated the impact (i.e., viability, density, and phenotype) of depositing cells on asurface submerged in cell culture media. It was determined that submersion of the microfluidic print head in cell media didnot alter the cell density, viability, or phenotype.. This article describes an in depth study detailing the impact of one of thefundamental components of a 3D microfluidic cell printer designed to mimic the in vivo cell environment. Development ofsuch a tool holds promise as a high-throughput drug-screening platform for new cancer therapeutics.