Collagen self-assembly on orthopedic magnesium biomaterials surface and subsequent bone cell attachment

摘要

Collagen monomer can form different structures on Mg biomaterial depending on the initial collagen monomer concentration. pH in the solution, assembly time, electrolytes, and the material surface roughness. The pH can change the electrostatic properties of collagen and final collagen structure. With the increase of collagen assembly time, small fibrils merge with adjacent fibrils forming thicker fibers. Materials with rough surface show stronger collagen adsorption ability. Initial cell attachment on RS of materials decreased independent of the composition of materials. AZ31 surface roughness and collagen structure did not affect cell proliferation for up to 7 days. Materials with high degradation rate may not change collagen assembly structure but can affect osteoblast cell proliferation. In the future study, the combined effect of those factors such as pH. material surface roughness, assembly time and electrolytes on collagen assembly, mineralization and bone cell interaction should be addressed.