In Situ Forming Gelatin-Poly(Ethylene Glycol)-Tyramine Hydrogels via Dual Enzyme of Horseradish Peroxidase and Gluose Oxidase

摘要

The in situ cross-linkable GPT hydrogels are successfully prepared via HRP-and GOx-mediated reaction, and the physico-chemical properties of the hydrogel matrices could be controlled by varying material parameters. The obtained results support our hypothesis that GOx can catalyze an in situ hydrogelation of GPT conjugates in the presence glucose and HRP. Taken together, we expect that the dual enzyme-mediated in situ hydrogelation can be used as a certain alternative to the previous conventional that has been widely used to prepare the enzymatically cross-linkable hydrogels.