Combining Human Monocytes in Co-culture with Vascular Smooth Muscle Cells Enhances Vascular Tissue Production under Biomechanical Stimulation

摘要

Combining monocyte co-culture and biomechanical stimulation had complementary effects on increasing VSMCs, and biomechanical stimulation further supported tissue deposition and enhanced mechanical properties. MDMs were shown to promote stimulatory growth factor release (FGF-2) from VSMCs, while also being polarized by D-PHI to an M2c-state over time. The latter is conducive to supporting cell/tissue growth. This study highlights the strategic use of MDM-co-culture in an appropriate biomaterial with immunomodulatory character (D-PHI). along with biomechanical stimulation, as an indirect approach to stimulate tissue regeneration.