Colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA Human Papillomavirus (HPV) immobilized on silicon dioxide (SiO2) thin film

摘要

This research studies the detection of Human Papillomavirus (HPV) by using a colorimetric method for visualizing biotin-labelled DNA probes hybridized to DNA HPV on silicon dioxide (SiO). In this study, SiO was used as substrate to check hybridization between HPV DNA probe and HPV DNA target. HPV is a virus that spreading by sexually transmitted that infected to human especially woman. High-risk HPV that stimulate cervical cancers (CC) is genotypes 16 and 18. Most 70% of CC are due to infection by these genotypes. The efficiency of colorimetric method depends mainly on the sequence of the specific oligonucleotide probe and the way they are attached to the support thin film. The conjugating procedure must be quick, strong by using covalent binding, and reproducible to show immobilization and hybridization process successful. The detection limit of target DNA has been estimated by using fluorescent detection.