MORPHOMETRIC ANALYSIS OF THE LOCATION AND ACTIVITY OF CYTOKINES IN THE TISSUE IMPLANT RESPONSE

摘要

The objective of this investigation was to evaluate the location and activity of cytokines in the fibrous tissue surrounding tricalcium phosphate (TCP) implants loaded with androgenic hormones. Sixteen animals in four experimental groups (n = 4/group) were implanted with one TCP implant each: Group Ⅰ (control), Group Ⅱ (testosterone), Group Ⅲ (dihydrotestosterone), and Group Ⅳ (androstenedione). At 90 days post-implantation, the fibrous tissue surrounding the implants were evaluated following staining with antibodies to IL-1β, IL-2, IL-6, and TNFa. Data were collected on the presence and distribution of cytokines within the fibrous tissue surrounding all four groups. IL-1β was primarily found intercellular and associated with fibroblasts and macrophages of Groups Ⅰ-Ⅲ. IL-2 was present in the extracellular matrix and was sporadically found on the surface of macrophages in Groups Ⅰ-Ⅲ. IL-6 was found primarily concentrated in the fibroblast and collagen rich portions of the fibrous tissue matrix in Groups Ⅰ-Ⅲ. TNF-a was present in the extracellular matrix of the fibrous tissue of all four groups and was strongly associated with fibroblast and macrophage rich areas. The results of this study confirm activity of cytokines on target cells and indicate their actions may vary in their effect within the fibrous tissue surrounding TCP implants loaded with androgens.