Calculating the Melting Temperature of Linker DNA

摘要

We created a program which addresses the intrinsic relationship between a DNA sequence and its subsequent melting temperature. The purpose of our program was to find the melting temperature of any given DNA sequence and return a DNA sequence which matches a desired melting temperature. Our algorithm assists users by providing a consistently error-free sequence generator to form the building blocks of DNA origami. With our algorithm, chemists and students alike can quickly and efficiently find the correct DNA sequence to match a desired melting temperature. Because the Chemistry department at Simpson College has invested heavily in DNA origami research, our program will be instrumental to their studies and eventual findings.