Measuring Organelle Shape in 3D in Stem Cells Cultured on Nanofiber Scaffolds

摘要

hBMSCs and their organelles had a smaller volume during culture on NF scaffolds as compared to SC planar substrates. However, the nuclei and peroxisomes occupied a significantly (P ＜ 0.05) larger fraction of the hBMSC cell volume during culture on NFs . Peroxisomes generate energy from catabolism of fatty acids and the increase in their cell volume fraction during NF culture could enable increased energy production. Likewise, the increased volume fraction of the nuclei for NFs may enhance output of mRNA available for translation and protein synthesis.