Sorting cells from fluorescent markers organization in confocal microscopy: 3D versus 2D images

摘要

We consider the problem of sorting cells from fluorescent markers spatial organization via single cell microscopy. This problem is important in bioimaging since this organization can reflect the healthy or pathological state of cells, the chromatin chain configurations or the spatial organization of DNA during main cell cycle. In this work, we address the question of the detection of spatial organization differences according to fluorescent markers density or distribution using textural features. We compare the performances by simulating 3D images and 2D images of the focal plane with various size of the point spread function of the confocal microscope, that is determined by varying the numerical aperture N A of the used objective lens.