Sustained Release of Bone Morphogenetic Protein-2 in Interpenetrating Composite Hydrogels for Promoting Skull Bone Regeneration

摘要

In this study, a two-step release platform of BMP-2 was prepared by utilizing Gel-SH/PEGDA IPN composite hydrogels containing GF carriers of Coa and GMP. In vitro osteogenic differentiation result demonstrated that BMP-2 released from both Coa and GMP carriers retains its bioactivity and that the use of carriers could be more clinically relevant than delivery/ injection of bolus BMP-2. Additionally, in vivo bone regeneration results indicated enhanced new bone formation in IPN composite hydrogels containing carriers of Coa and GMPs. Moreover, in vivo skull bone regeneration could be modulated by (1) the selection of a carrier system, and (2) release profile over time and release amount of BMP-2 in the certain defect volume. Therefore, physiologically optimal levels of BMP-2 are one of the more critical parameters for the precise control and augmentation of skull bone repair procedures. Taken together, our results suggest that Gel-SH/PEGDA IPN composite hydrogel could be used as scaffold materials for GBR and that Coa and GMPs could be utilized as promising BMP-2 carriers in bone tissue engineering.