Efficient Aortic Valve Cell Seeding into Decellularized Pericardial Membrane: Advancements into Manufacture of Engineered Valve Tissue for the Desing of 'off the shelf', Living, Valve Bioimplants

摘要

Conclusions: Our data show, for the first time, the employment of a direct perfusion bioreactor to seed cell-free pericardium patches with aortic valve-competent cells. While this demonstrates the utility of our approach to favor cell seeding of a the animal-derived pericardium with cells with an unprecedented efficiency, it has also important implications for manufacturing living valve bioimplants with a controllable and reproducible system. References: Vinci MC, PLoSOne. 2013; 8(5):e64769.