First Trimester Human Umbilical Cord Perivascular Cells Accelerate and Enhance Network Formation of Human Blood Outgrowth Endothelial Cells in 3D Matrigel Environments

摘要

Conclusions: BOECs seeded on or embedded within Matrigel? demonstrate tubular network formation potential. BOECs have shown similar network formation potential in GFR Matrigel?. Preliminary video imaging demonstrates that FTM HUCPVCs home to nodes of BOEC networks. FTM HUCPVCs establish strong interactions with BOEC cells that disrupt 2D endothelial networks. Such an effect was not observed in transwell co-cultures (i.e. not paracrine co-cultures), that suggests that paracrine factors are not responsible for 2D endothelial networks disruption. 3D Matrigel? embedded BOECs form extensive networks throughout the culture. Co-embedded FTM HUCPVCs form connections with BOEC networks. Network formation is accelerated in 3D BOEC cultures co-embedded with FTM HUCPVCs compared to 3D cultured BOECs alone. Overall, BOECs as an endothelial cell source in combination with efficient supporting cells such as FTM HUCPVCs can be promising candidates to produce vasculature inducing injectable biomaterials. Further work is required to determine the nature of the strong interactions between BOEC networks and FTM HUCPVCs in direct co-cultures.