Modular, Integrin Ligand-Specific Immune Organoids Differentially Regulate Ex Vivo B Cell Activation

摘要

Conclusions: In this study, we present a biomaterials-based modular immune organoid to understand the role of the lymphoid niche, particularly integrin ligands, in the development of early GC-like phenotype. This phenotype developed in a concentration dependent and integrin ligand specific manner. Presentation of α4β1-binding ligand clustered integrins and BCR, leading to a more robust GL7+ phenotype. Organoids that presented both ligands were similar to RGD-organoids, suggesting α_vβ_3 may play a dominating or regulatory role in the niche. We anticipate that the ability to drive immune reactions ex vivo will allow us to reproduce selective aspects of GC events with tunable parameters for a better understanding of B cell maturation and antibody secretion.