Combination of Pathogen-mimicking Polymer Particles and an Injectable, Synthetic Immune-Priming Center (sIPC) Significantly Enhances Cellular and Protective Immunity in Murine Models of Cancer

摘要

Polymer based, pathogen-mimicking micro- and nanoparticles capable of delivering pDNA/protein antigens along with multiple TLR ligands on the surface of the same particles were developed. These particles can load tumor antigens and adjuvants both separately as well as simultaneously on a single particle with high loading efficiencies. This surface loaded particulate system is easy to formulate and the doses of antigens and various nucleic acid based TLR ligands per particle can be controlled. The paniculate formulations were able to immunologically protect mice from lethal A20 lymphoma and Melanoma-OVA tumor challenge in prophylactic and therapeutic settings. Thus, a polymer based multifunctional versatile paniculate delivery system has been developed for cancer immunotherapy. A comparative immunotherapeutic efficacy of polymer micro vs nanoparticles study is in progress.