Precisely integrating multiple different protein ligands into supramolecular assemblies

摘要

Fusion proteins having a β-sheet fibrillizing domain integrated into nanofibers of synthetic β-sheet fibrillizing peptides in a βTail-dependent manner. Multiple different βTail fusion proteins co-assembled into Q11 nanofibers, with each protein integrated at a precisely defined dose. Nanofibers with an integrated βTail fusion protein acted as self-adjuvanting vaccines. Together, these observations suggest that this versatile approach is likely to enable development of multi-antigen subunit vaccines with precisely tunable molecular composition.