Decreasing bacterial colonization around the Intraosseous Transcutaneous Amputation Prosthesis without inducing cytotoxicity to fibroblasts using hydroxyapatite, silver and fibronectin

摘要

The HAAg surfaces tested in this study have antibacterial activity compared to HA controls before and after preconditioning in fetal calf serum. Preconditioning removes the detrimental effect of Ag on fibroblast viability. The clinical significance of the presence of cytotoxicity before preconditioning is unknown, but it is likely there would be a delay in soft tissue attachment. A study of the effect of preconditioning for longer time periods would be useful to determine whether the antibacterial activity of these HAAg surfaces is maintained longterm. Challenges with other bacteria, including Pseudomonas Aeruginosa and bacteria from a clinical pin site infection will be carried out. Additionally, an in vivo study of antibacterial activity and soft tissue attachment using an established transcutaneous ovine model is planned.