Lyophilized Poly(ethylene glycol-b-(diniethylaminoethyl methacrylate-co-butyl methacrylate))-DNA Nanoparticles for Nonviral Gene Therapy

摘要

Novel poly(EG-b-(DMAEMA-co-BMA))-pDNA nanoparticles were shown to be stable after lyophilization and incubation in PBS and efficiently transfect cells in vitro. Except for 50L, complexes with N/P ratios of 10 and 20 had smaller sizes after lyophilization than Hpofectamine2000 and PEI and higher transfection efficiency than PEI. The time constants for aggregation determined from the exponential fits provide quantitative evidence for the increased stability of poly(EG-b-(DMAEMA-co-BMA)) complexes compared to Iipofectamine2000 and PEI. Future work will involve incorporating lyophilized poly(EG-b-(DMAEMA-co-BMA))-pDNA nanoparticles into tissue engineering scaffolds for in vivo gene therapy applications.