Development of a Poly (lactic acid) Poly(ethylene glycol) Nanoparticle for Delivery of Vitamin D_3 for Severe Asthmatics

摘要

The PLA-PEG block copolymer was successfully synthesized and purified for HASM cell application, as verified by HNMR and the Vi-Cell analyzer. The molecular weight of the polymer averaged 600 g/mol. SEM and DLS verified formation of the micelle-like nanoparticles consistent spherical shape and size. Additionally, HASM cells treated with the nanoparticle demonstrated transcription of CYP24A1 similarly to Vit D. Since it has been proven that synthesis of PLA-PEG nanoparticle is achievable and cell viability is good, optimizations of the copolymer as a delivery agent for Vitamin D in HASM warrants further studies.