Utilizing Biochemical Analyses as a Predictor for Structural Alterations of Fibroblasts Exposed to Adhesives in Combination with Nifedipine and Periodontal Pathogens

摘要

Many cofounding factors affect the health of the oral cavity. Clinicians must be aware of medications that can affect the periodontium. In addition, there must be a thorough knowledge of interactions that may be resultant due to interactions between a disease state and medications. Two of the most common infections of the oral cavity are dental caries and periodontal disease. Each alone negatively impacts the health of patients, however, in combination the effects may be detrimental. Previous studies have examined the outcomes of fibroblast morphology when exposed to the combination of dental adhesives and Nifedipine. The aim of the study was to utilize biochemical analyses as a predictor of structural alterations of fibroblasts exposed to adhesives in combination with Nifedipine and the periodontal pathogen, Porphyromonas gingivalis. The fibroblasts were exposed to 0.1g of dental adhesives (PMMA, OptiBond??, and Prime & Bond??) in combination with Porphyromonas gingivalis Lipopolysaccharide (2??L) and Nifedipine (10??L) for 48 Hours. Biocinchonic acid assay (BCA), reduced glutathione, and lactate dehydrogenase were the biochemical analyses utilized. When assessing metabolic activity, oxidative stress levels, and membrane damage, there were significant differences amongst the experimental groups compared to the control (PP