Challenges In The Measurement Of Protein Electrophoretic Mobility

摘要

Dynamic Light Scattering (DLS) is a widely implemented technique for characterization of proteins and their formulations that allows the determination of molecular size and protein stability, while Electrophoretic Light Scattering (ELS) can be used to measure protein mobility, which has been identified as a promising indicator of formulation stability, viscosity and behaviour. Measurement of electrophoretic mobility requires the application of an electric field to the sample, which can stimulate aggregation that could reduce the reliability of the measurement. A new DLS/ELS measurement technique, the Diffusion Barrier Method, will be described that protects the protein by isolating it from the cell electrodes, which ensures that the measured electrophoretic mobility is that of the protein of interest rather than aggregates that have formed during the measurement.