Cellular uptake and cytotoxic effects of broccoli phytochemicals based gold nanoparticles (B-AuNPs): Enhanced cancer therapeutic efficacy

摘要

The interaction of phytochemicals from Broccoli with gold salt results in dual reduction and surface capping to produce well-defined stable and biocompatible gold nanoparticles (B-AuNPs). The core sizes of B-AuNPs is 15±2 run as measured from TEM techniques which was also corroborated by CPS technique. Hydrodynamic diameter of B-AuNPs is 100±5 nm, suggesting that cocktail of broccoli phytochemicals are capped on gold nanoparticles. The highly -ve zeta potential of -29.0 mV indicates that the particles are sufficiently stable. The effect of B-AuNPs on various cancer cell lines showed significant cytotoxic effect. The cellular localization of B-AuNPs against cancer cell lines was evaluated using dark field optical microscopy and TEM image analysis. Cytotoxic results and tumor cell affinity suggest that the unique synergistic cocktail effect of B-AuNPs may provide new opportunities for generating biocompatible AuNPs for applications in molecular imaging and therapy.