We have successfully fabricated N-isopropylacrylamide (NIPAM) core-shell microcapsules in a hard polymer-based microfluidic chip. The demonstrated strategy leads to preparing monodisperse microcapsules for encapsulating Bacillus thuringiensis (BT) spores. The viability of the microencapsulated spores was confirmed by the culture of vegetative cells after the germination. This in situ microencapsulation methodology provides a unique method for synthesizing bioactive hollow polymeric microcapsules.
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