DETECTION ON THE EXPRESSION OF TANS-GENE IN RICE PLANT BASED ON HYPER-SPECTRAL REMOTE SENSING TECHNIQUE

摘要

As rapid, efficient, non-destructive, non-polluting etc., spectroscopy in laboratory to identify trans-gene crops has been widely used. This study aims to identity the expression of trans-gene in given rice plant samples in strictly controlled contrast experiment. To avoid external noise caused by temperature change and water-loss, field spectrum had been collected with ASD field spectrometer in natural state. The study calculated mean spectrum of samples as main data set analyzed which were controlled by inner clustering coefficient to ensure data quality. By mean spectrum, Noise from random distinctions in few individual cultivators which could not be expressed in the class stably could be weakened even filtered. With help of parameters, such as red edge, green peak, this study gave qualitative spectral differences between transgenic samples and their parents. The results show the trans-genes in rice plant had been expressed and made spectral difference between samples. Moreover, it is found that genes transferred into had difference spectral influence intensity at different part of sample leaves. We gave quantitative analysis about these influences. These conclusive results demonstrate that hyper-spectral remote sensing technique has a good prospects and application potential in trans-gene expression detection and monitoring, especially in plant breeding process.