Design of Surface Electrode for Measurement of Electric Impedance of Arrangement of Cells Oriented on Micro Striped Pattern

摘要

The surface electrode has been designed for measurement of the electric impedance of biological cells oriented on the micro striped pattern in vitro. Several parallel lines of micro rectangular grooves (1μrn depth, 3 urn width, and 3μm interval) have been made between a pair of the surface titanium electrodes at the surface of the scaffold of glass by the photolithography technique. Two types of arrangements of lines are designed at the micro ridges: parallel, and perpendicular to the electric line of force. Variation has also been made on the shape of surface electrodes (thickness; 0.2 μm): I-shape and T-shape. Two kinds of cells were alternatively cultured on the micro-patterned scaffold: C2C12 (mouse myoblast cell line), and 3T3-L1 (mouse fat precursor cells). The electric impedance between electrodes was measured for 24 hours with the sinusoidal electric waves (frequency, 1 kHz