Reconstruction of Extracellular Matrix Microenvironment to Facilitate Expansion and Chondrogenesis of Synovium-derived Stem Cells

摘要

In this study, we successfully engineered an in vitro 3D stem cell niche using SDSC-deposited ECM for improving SDSC proliferation while enhancing SDSC sternness toward chondrogenesis. In contrast, SDSCs grown on plastic flasks displayed diminished replicative capacity. We demonstrated that the 3D cell-deposited ECM provides the proper vitro expansion of SDSCs, not only promoting self-renewal ability but also improving chondrogenic potential, and the novel 3D expansion system greatly improves cell-based cartilage regeneration.