Host-Guest Interaction Mediated Assembilies: Supramolecular Nanoparticles for Paclitaxel/siRNA Co-delivery

摘要

To achieve synergistic effect of anti-tumor agent and therapeutic gene, a novel type supramolecular nanoparticle which perform co-delivery of paclitaxel and siRNA was synthesized via host-guest interaction. In the self-assembly complex (termed as PC/Ada-PTX), the cationic PEI_(600)-CD (PC) vectors composed of β -cyclodextrin (β-CD) and low-molecular-weight polyethylenimine (PEI, Mw 600) served as the host and the adamantane-conjugated paclitaxel (Ada-PTX) as the guest. The cytotoxicity of the PC/Ada-PTX complex was much lower than that of PEI 25 kD in SKOV-3 cell line. Functioning of paclitaxel accompanied by siRNA which silenced the survivin gene exhibited novel lethality to the tumor cells, which is confirmed by significant up-regulation of cell-cycle arrest and cell apoptosis. By hypodermic injection of the SNP into the tumor-bearing nude mice, it was found that the SNP remarkably enhanced the anti-tumor effect. The host-guest assembled SNP should have great potential applications in cancer therapy.