Influence of Nanofiber Diameter on ERK and p38 Activation in Osteoblastic Cells

摘要

The results demonstrate that etching provides excellent control over the fiber diameter and it is possible to reproducibly reduce the diameter from 2.416±0.100 μm to 0.552±0.047 μm. The in-cell western study indicates that the fiber diameters provide the required mechanical stimulus to affect the activation of ERK and p38 MAP kinases. These results underscore a mechanism by which cellular response to different fiber diameters can lead to altered levels of cell proliferation, associated with ERK, and early osteoblastic differentiation, associated with p38. In conclusion, fiber diameter can be utilized to direct the activation of ERK and p38, potentially stimulating osteoinduction.