Thiol-Ene Clickable PEG Based Thermoresponsive Hyperbranched Copolymer for In Situ Crosslinking Hybrid Hydrogel

摘要

PEGMEMA-MEO_2MA-PEGDA copolymers with both thermoresponsive and chemical cross-linkable behavior were synthesized via in-situ DE-ATRP. The polymer solution could form a thermal gel at body temperature, while the chemical gelation occurred less than 5 minutes once mixing with thiolated HA. LIVE/DEAD~® assay was employed with 3T3 and ADSCs after one week in culture, which was indicated that with very few dead cells, both cell types survived well in 3D hydrogels. This new hydrogel system has shown promise as an injectable system for tissue engineering application.