Biomimetic Sensors in Medicine and Biology - Detection of Bioparticles

摘要

Mass- sensitive devices and interdigital capacitors were combined to receptor layers to design sensors for bioanalytes. In this way proteins, viruses cells up to pollen can be detected. The bioanalytes ranges from a few nano-meter to many micrometer. The interaction scheme between analyte and receptor layer is based on intermolecular forces and host-guest chemistry, which recognizes the geometrical properties of bio-particles. The realization of the sensor coatings is performed by molecular imprinting. Thus, the analyte to be detected is pressed into a pre-polymer to create a cavity for re-inclusion. Furthermore, a second pre-polymer is casted in the hollow generating a "plastic" analyte which will used again to generate sensor coatings by patterning. This can also be performed in this way by applying natural antibodies to transfer biorecognition capabilities to synthetic receptors.Human insulin and its derivatives are monitored down to the physiological limit. This strategy allows the detection of e.g. human rhino viruses, even different serotypes can be distinguished. The sensor signals differentiate between strains of cells. In spite of the high cell wall flexibility of erythrocytes blood group typing can be performed, even in respect to subgroups.