Highly sensitive bioassays are required for biological, medical, and pharmaceutical applications. In this paper, a highly sensitive immunoassay performed using a microstructured surface have been described. The surface was fabricated by etching a silicon wafer coated with microparticles. Bovine serum albumin and fluorescence-labelled anti-BSA antibodies were adhered onto the microstructured surface. Next, the sensitivity of this assay was evaluated by microscopic analysis of the fluorescence intensity of the surface. The results indicate that use of a microstructured surface greatly increases the sensitivity of the assay and that such surface can be used for several applications in the medical and biological fields.
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