BUTANOL PRODUCTION FROM CELLULOSE-CONTAINING SOURCES BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION USING IMMOBILIZED CELL BIOCATALYSTS

摘要

the main purpose of the work is demonstration of possible application of biocatalysts developed on thebase of genus Clostridium cells catalyzing acetone-butanol-ethanol (ABE)-fermentation and entrapped into PVAcryogel for the production of biobutanol from various industrial and agricultural cellulose-containing wastes as mainsources in the frame of simultaneous saccharification and fermentation process. Cellulose-containing wastes ofindustry and agriculture were applied as main substrates for immobilized cells of microorganisms producing butanol.The content of immobilized biocatalysts producing ABE-fermentation was optimized. The positive influence ofsimple gas-stripping techniques on butanol production with immobilized cells was demonstrated. The effectivesimultaneous saccharification and fermentation of various cellulose-containing wastes to biobutanol was performedusing complex of cellulolytic enzymes and immobilized cells Clostridium acetobutylicum.