Trapping of Jurkat Cells using a Single-Beam Optical Tweezer and a Micromachined Fresnel Zone Plate

摘要

This paper describes the use of a singlebeam optical tweezer to measure the interactive forces between two Jurkat cells (a white blood cells diseases with a diameter of 14 urn). Experimental indicate that a laser power of l.5 mW was sufficient to trap individual Jurkat cells. The laser power of 10.0 mW generates a dragging force of 0.12 pN. Separating two mutually attached Jurkat cells requires a laser power of 4.0 mW. Experimental results that as the adhesion time of the two Jurkat cells is increased, the separation power also increases. A single-beam optical tweezer was integrated with a micromachined Fresnel zone plate. The trapping of Jurkat cells in a culture solution using a micromachined Fresnel zone plate was demonstrated. The advantages of the Fresnel zone plate are its much smaller thickness and weight, the flexibility of the lithographic process, and the ease with which they can be integrated into microfluidic chips.