Miniaturization has brought important advantages to separation technologies; such as reduced sampleand reagent consumption; higher resolution and sensitivity, low cost and shorter processing time.Dielectrophoresis (DEP) is an extensively employed micro analytical technique with great potential forthe manipulation of a wide array of bioparticles. Insulator-based DEP (iDEP) is a dielectrophoreticmode where nonuniform electric fields are created employing insulating structures.In this work, an evaluation of the selectivity of an iDEP microdevice by employing mixtures ofpolystyrene nano and microparticles is presented. Experimental and mathematical modeling work wascarried in order to assess the selectivity of an iDEP glass microdevice to immobilize only the largeparticles from a binary mixture. The effects of particle concentration, concentration ratio, size ratio,and magnitude of the applied electric potential (200 to 600 V) on the microdevice selectivity werestudied. The results demonstrated that high selectivity can be obtained with iDEP.
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