Nondestructive Quantification of Permeability of Hyperosmotic Agent in Normal and Tumor Tissues

摘要

Noninvasive tumor imaging could lead to the early detection and timely treatment of cancer. Previous investigations have suggested that optical coherence tomography (OCT) is an ideal diagnostic tool distinguishing normal tissues from tumor tissues based on structural imaging because of the high resolution. In the study, the capability of OCT for functional imaging of normal and tumor tissues based on time and depth resolved quantification of the permeability of biomolecules through these tissues is investigated. An OCT system at 830 nm central wavelength was used in this study. Diffusion of 20% aqueous solution of glucose was monitored and quantified in normal stomach tissues and tumor tissues using OCT. The orthotopic graft model of gastric cancer in nude mice was used. Permeability coefficients were calculated as a function of time and tissue depth. The permeability coefficient was (9.44±0.42) ×10~(-6)cm/s in normal stomach tissues and (5.32±0.17)×10~(-5) cm/s in tumor tissues. The tumor tissues had a higher permeability coefficient compared to normal tissues. From the experimental results, it is found that the accurate and sensitive assessment of the permeability coefficients of normal and tumor tissues offer an effective OCT image method for clinical diagnosis and detection of tumor tissues.