A NOVEL TECHNIQUE FOR THE DIRECT DETECTION OF DNA HYBRIDIZATION

摘要

We propose a novel technique to detect DNA hybridization. The technique involves measuring scattered light under surface plasmon resonance (SPR) conditions. We have shown that the maximum scattering angle correlates with the traditionally employed reflection minimum. Measuring of scattered light under SPR conditions has all the functional advantages of the SPR technique. In addition, the proposed technique simplifies device design, increases the dynamic range of analysis, and integrates data with those from surface-plasmon field-enhanced fluorescence spectroscopy. We demonstrate the technique by showing direct registration of 20 bases oligonucleotide hybridization. Also we have investigated the amplification effect of 20nm gold nanoparticles (GNP) on the oligonucleotide immobilization process.