Stability Model Construction of Long-Circulating Immuneliposome Targeting to Dendritic Cell Surface Molecule DEC-205 and Analysis of its Biological Characteristics

摘要

Kinetic stability and coalescence stability theory of multi-dispersal systems have been advanced to construct the stability model of long-circulating immuneliposome targeting to dendritic cell surface molecules DEC-205 (anti-DEC-205 iLPSM). Then its physicochemical properties as well as biological characteristics is tested.The optimized liposomes show stable physicochemical properties when stores at 4°C for 7 days.Under this condition,liposome size and size distribution do not noticeably change.Cumulative leakage rate of FITC-dextran from liposomes is less than 7%.Anti-DEC-205-immunoliposomes (anti-DEC-205 iLPSM),as drug carriers,can specifically recognize dendritic cells and deliver FITC-dextran into the cytoplasm.The construction of an anti-DEC-205 iLPSM model lays solid foundations for further work researching immune response after antigen targeting to DEC-205 receptors in vivo.Anti-DEC-205 iLPSM could become a new type of DC vaccine.