The effect of remnant cationic dendrimers on RNA purification

摘要

We examined RNA isolation using the common method of monophasic lysis from human macrophage-like cells (U937) that had been exposed to dendrimers and DNA/dendrimer complexes using gel electrophoresis. We found that poly(amidoamine) (PAMAM)dendrimers strongly altered the mobility of RNA in the gels. In addition, the extent of dendrimer-induced alteration in RNA mobility was directly dendrimer-generation-dependent: the alteration was greater with higher-generation dendrimers. We also found that DNA/dendrimer complexes at higher dendrimer-to-DNA ratios interacted with RNA after isolation. Because RNA isolation is routinely used for biological applications, such dendrimer-induced alteration in RNA mobility should be accounted for in the further processing of RNA-related applications.