TITLE:Synthesis and Targeted Radiotherapeutic Assessment of ~(188)Re(V)DMSA Microspheres In GliomaCell Lines

摘要

99mTc(V) Dimercaptosuccinic acid (DMSA) has been well documented as a well-known tumor-seeking agent .The attachment of a β-emitter / higher energy γ- emitter in lieu of positioned Tc-99m of DMSA molecule, loaded in a polymeric microspheric delivery system may be utilized theoretically as therapeutic agent for neurogenic/ neuroendocrine tumors. Studies were therefore conducted using bioabsorbable poly lactic -co glycolic acid (75:25) as synthesized polymeric microspheres loaded with Re-188 tagged dimercaptosuccinic acid. A solvent evaporation technique was employed to encapsulate DMSA in PLGA micro spheres. Drug loading were initially evaluated using I.R. spectroscopy & spectophotometric analysis. Drug release kinetics indicated that the total amount of DMSA released and the release rate were directly dependent on loading percentage. Radiolabelling efficiency of the tagged polymer was more than 98%. The cold & Re-188 labeled DMSA- PLGA polymeric microspheres were incubated separately with human Glioma cells in cultured medium for the assessment of its in-vitro kinetics on cell lines. The presence of microspheres nearer to its action sites was well demonstrated by electron microscopy.