Analysis of genetic diversity in rapeseed

摘要

Genetic diversity of 64 rapeseed varieties of Brassica napus L. was analyzed by RAPD. 11 polymorphic primers out of 180 random primers were selected. 120 bands were amplified and 65 bands were polymorphic (54.2%). The number of polymorphic bands of Primer 1946 was the maximum (8 bands). That of Primer 1956 was the minimum (4 bands). The 64 materials were divided into five groups by cluster analysis. The majority of materials in the first, the fourth and the fifth groups were landraces from Sichuan. The materials in the second group were landraces from Sichuan and varieties bred in Sichuan. The materials in the third group were varieties introduced from other countries and landraces from Sichuan. The genetic relationship among different materials displayed in the cluster tree indicated the genetic difference between the landraces of B. napus L. from Sichuan and varieties of B. napus L. introduced from other countries was obvious. That was the same between the landraces from Sichuan and approved varieties in China. The genetic diversity in the landraces from Sichuan was very abundant.